Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.274 | - |
NAD+ | at pH 10.0, temperature not specified in the publication | Bacillus cereus | |
0.652 | - |
(S)-3-hydroxyisobutyrate | at pH 10.0, temperature not specified in the publication | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32700 | - |
- |
Bacillus cereus |
65000 | - |
gel filtration | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyisobutyrate + NAD+ | the enzyme is very specific to (S)-3-hydroxyisobutyrate | Bacillus cereus | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
3-hydroxypropionate + NAD+ | - |
Bacillus cereus | 3-oxopropanoate + NADH + H+ | - |
? | |
L-serine + NAD+ | - |
Bacillus cereus | 3-oxo-L-alanine + NADH + H+ | - |
r | |
additional information | no activity with NADP+, DL-glycerate, (R)-3-hydroxyisobutyrate, L-threonine, D-threonine, and D-serine | Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32700, calculated from amino acid sequence | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
HIBADH | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.1 | - |
NAD+ | at pH 10.0, temperature not specified in the publication | Bacillus cereus | |
24.1 | - |
(S)-3-hydroxyisobutyrate | at pH 10.0, temperature not specified in the publication | Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | - |
Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.1 | - |
(S)-3-hydroxyisobutyrate | at pH 10.0, temperature not specified in the publication | Bacillus cereus | |
55.1 | - |
NAD+ | at pH 10.0, temperature not specified in the publication | Bacillus cereus |